GGPPS (geranylgeranyl diphosphate synthase) directed single-point mutant protein GGPPS-154

• Main Authors: XU SHAOYAN, DONG CHEN, WEI PAN, LI FENG, MAO JIAQI, YANG NINGNING, WANG RAN, JIN LIFENG, FENG MINGXING, DU FEI
• Format: Patent
• Language: Chinese; English
• Published: 14.08.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention belongs to the technical field of tobacco genetic engineering, and particularly relates to GGPPS (geranylgeranyl diphosphate synthase) directed mutant protein. The 154th site, the 161stsite and 218th site of existing GGPPS protein are located in a catalytic pocket of enzyme, and the 209th site and the 233rd site are located on the surface of an enzyme molecule. On the basis of the five sites, the invention provides the GGPPS series directed mutant protein. The GGPPS series directed mutant protein comprises series single-site mutant protein, double-site mutant protein, three-sitemutant protein, four-site mutant protein and five-site mutant protein. With adoption of a CAST technology, a small and fine mutant library is constructed, and on the basis of further screening and directed evolution demands, amino acid mutation type of the specific sites are analyzed in detail. A preliminary experiment result indicates that after amino acid of the specific sites is mutated, the synthetic amount of beta-ca