Culture medium for culturing MDCK cell line and preparation method of culture medium

• Main Authors: ZHAN XUANZI, ZHANG XIAONAN, ZHANG YUNLI, ZHENG CAILI, WANG TUHUO, KONG FENGYING, XIE YUHONG, LIU DECHENG
• Format: Patent
• Language: Chinese; English
• Published: 14.08.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the field of cell culture, in particular to a culture medium for culturing an MDCK cell line and a preparation method of the culture medium. The culture medium comprises basicmetabolic nutrients, trace elements, vitamins, salts, liposomes, additives and the like. The preparation method is simple, low in cost and convenient to operate. The prepared culture medium can support MDCK cell suspension culture and promote MDCK cell growth and is high in safety, and the cultured MDCK cell line can be applied to production of avian influenza vaccine. 本发明涉及细胞培养领域，特别涉及用于培养MDCK细胞系的培养基及其制备方法，包括基础代谢营养物类、微量元素类、维生素类、盐类、脂质体、添加物等。制备方法简单，成本低，便于操作。制得的培养基能支持MDCK细胞悬浮培养，促进MDCK细胞生长，而且安全性好，培养的MDCK细胞系可应用于禽流感疫苗的生产中。