WNT/SFRP COMPLEXES, WNT-CONTAINING COMPOSITIONS, WNT-EXPRESSING CELLS, AND METHODS OF MAKING, PURIFYING, AND USING SAME

• Main Authors: XIONG LIWEN, BI MING, TRACY CAMRIN, PERSON ANTHONY
• Format: Patent
• Language: Chinese; English
• Published: 07.08.2020
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

This disclosure describes isolated protein complexes including a Wnt and a sFRP; compositions including a Wnt; a cell overexpressing a Wnt and a sFRP; compositions including a cell overexpressing a Wnt and a cell overexpressing a sFRP; methods of making the protein complexes, compositions, and cells; and methods of using the isolated protein complexes, compositions, and cells. This disclosure further describes methods of forming a complex including a Wnt and a sFRP and methods for isolating a Wnt. Also described herein are methods that may be used to purify a Wnt without the use of a detergent. 本公开内容描述了包括Wnt和sFRP的分离的蛋白质复合物；包括Wnt的组合物；过表达Wnt和sFRP的细胞；包括过表达Wnt的细胞和过表达sFRP的细胞的组合物；制备蛋白质复合物、组合物和细胞的方法；以及使用分离的蛋白质复合物、组合物和细胞的方法。本公开内容进一步描述了形成包括Wnt和sFRP的复合物的方法，以及用于分离Wnt的方法。本文还描述的是可以用于纯化Wnt而无需使用去污剂的方法。