Method for determining components of freeze-dried powder injection containing multiple vitamins
The invention belongs to the technical field of analysis, and relates to a method for determining components of a freeze-dried powder injection containing multiple vitamins. According to the invention, the method comprises the steps: adopting the high performance liquid chromatography, wherein the c...
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Main Authors | , , , |
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Format | Patent |
Language | Chinese English |
Published |
07.08.2020
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Subjects | |
Online Access | Get full text |
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Summary: | The invention belongs to the technical field of analysis, and relates to a method for determining components of a freeze-dried powder injection containing multiple vitamins. According to the invention, the method comprises the steps: adopting the high performance liquid chromatography, wherein the chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a chromatographic column filler; a potassium dihydrogen phosphate solution containing 0.05%-2% of triethylamine is used as a mobile phase A, methyl alcohol is used as a mobile phase B, and gradient elutionis conducted according to a certain procedure; the flow rate is 0.5 mL/min to 1.5 mL/min; the column temperature is 20-35 DEG C; the temperature of the sample injector is 2-25 DEG C; the sample size is 20 to 80mu L; and the detection wavelengths are 200 nm, 265 nm, 325 nm, 285 nm and 448 nm. According to the determination method disclosed by the invention, the antioxidant is added in a sample preparation process, so a sam |
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Bibliography: | Application Number: CN202010454802 |