Method for quantitatively detecting specnuezhenide content in kidney-tonifying and pulse-harmonizing prescription by liquid chromatography-mass spectrometry

• Main Authors: CHEN KAI, QI MIN, LIU AIMING, WANG FANG, SUN JUNMEI, LIU XIAOYU, LU HAIYAN, WANG YINGCHUN, LI XIAOLIANG, ZHU XUEFEN, MA LIMING, XU LINGLING, WANG GONGXIA
• Format: Patent
• Language: Chinese; English
• Published: 04.08.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for quantitatively detecting a specnuezhenide content in a kidney-tonifying and pulse-harmonizing prescription by liquid chromatography-mass spectrometry. In liquid chromatography, a mobile phase is composed of acetonitrile (containing 0.1% of FA) and water (containing 0.1% of FA), and gradient elution is adopted. A mass spectrum adopts an electrospray negative ionsource and a multi-reaction monitoring analysis mode (MRM); and according to the method, a linear relation of specnuezhenide in a range of 100-5000ng/ml is good. Compared with an HPLC method, a pretreatment method of a test solution is simple to operate, short in operation time, high in detection method sensitivity, high in specificity and short in analysis time, and can be used for measuring thecontent of specnuezhenide and controlling quality of the kidney-tonifying and pulse-harmonizing prescription. 本发明公开了一种液质联用定量检测补肾和脉方剂中特女贞苷含量的方法。所述液相色谱中，流动相由乙腈(含0.1％FA)和水(含0.1％FA)组成，采用梯度洗脱。所述质谱采用电喷雾负离子源，多反应监测分析模式(MRM)；该方法中特女贞苷在