Method for screening dairy cow ketosis resistance molecular marker and application thereof

• Main Authors: LI RONGLING, JIANG QIANG, BAI JIACHEN, WANG JINPENG, WANG XIUGE, GAO YAPING, YANG CHUNHONG, WEI XIAOCHAO, LIU WENHAO, HUANG JINMING, ZHANG YARAN, JU ZHIHUA, GAO YUNDONG
• Format: Patent
• Language: Chinese; English
• Published: 04.08.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention provides a method for screening a dairy cow ketosis resistance molecular marker and application thereof, and belongs to the technical field of molecular genetic biology. Healthy and ketosis cows are taken as samples, chip sequencing, data filling, selection signal analysis, genome-wide association analysis and the like are carried out, and the key gene APOA1 influencing the ketosis resistance of the China Holstein cattle is screened out. By screening in an APOA1 gene promoter region, a single nucleotide polymorphism site g.-572 A being greater than G is obtained; through correlation analysis, promoter activity analysis and other methods, the concentration of beta-hydroxybutyric acid in blood of the GG genotype dairy cow is significantly lower than that of beta-hydroxybutyricacid in blood of AA genotype dairy cow, and the promoter activity of GG genotype individuals is significantly higher than that of AA genotype individuals. By the technical scheme, genotyping is conducted on the APOA1 gene pro