Soybean DNA fingerprint construction method and application thereof

• Main Authors: RUAN CHANGQING, LI ZHIJIANG, NIU JIANGSHUAI, ZHANG DONGJIE
• Format: Patent
• Language: Chinese; English
• Published: 31.07.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of soybean DNA fingerprints, in particular to a soybean DNA fingerprint construction method. The method comprises the following steps of S1, screening SSRprimers; S2, analyzing genetic diversity of soybeans, wherein soybean genome DNA extraction is performed, PCR amplification and detection are performed, a PCR reaction procedure is performed, capillary electrophoresis detection is performed and data statistics and analysis are performed; and S3, constructing a DNA fingerprint. SSR molecular markers are used for analyzing soybean resources collected in the Heilongjiang region, SSR molecular marker fingerprints of germplasm resources are constructed, and a theoretical basis is provided for germplasm preservation and screening of the Heilongjiangsoybeans. 本发明涉及大豆DNA指纹图谱技术领域，尤其涉及一种大豆DNA指纹图谱构建方法，包括以下步骤：S1、SSR引物的筛选：S2、大豆的遗传多样性分析：大豆基因组DNA提取；PCR扩增及检测；PCR反应程序进行；毛细管电泳检测；数据统计与分析；S3、DNA指纹图谱的构建，本发明利用SSR分子标记对黑龙江地区采集的大豆资源进行分析，构建各种质资源的SSR分子标记指纹图谱，为黑龙江大豆种质保存及筛选提供理论基础。