Method for preparing peptide treated by protein hydrolysis

• Main Author: ANDREAS RUMMEL
• Format: Patent
• Language: Chinese; English
• Published: 28.07.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to the invention titled 'method for preparing peptide treated by protein hydrolysis', which belongs to the field of protein engineering. Specifically, the present invention relates to a novel peptide with proteolytic activity and various applications of the peptide (and others) in screening and preparation methods. According to the present invention, the technical problem ofeffectively converting a single-chain precursor CNT into a truly mature cleavage product (i.e., a double-chain neurotoxin) is solved by providing a protease with a specific sequence. 本发明涉及发明名称为"用于制备经蛋白水解处理的多肽的方法"的发明，其属于蛋白质工程领域，具体而言，本发明涉及一种具有蛋白水解活性的新型多肽以及所述多肽(及其它)在筛选和制备方法中的多种应用，本发明通过提供具有特定序列的蛋白酶而解决了将单链前体CNT有效转换成真正成熟的切割产物(即双链神经毒素)的技术问题。