Mulberry calcium dependent protein kinase as well as coding gene and application thereof

• Main Authors: DU QIUXIA, LI YANG, ZHENG DANYAN, GUO PENG, ZHAO WEIGUO, SHI YISU
• Format: Patent
• Language: Chinese; English
• Published: 28.07.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses mulberry calcium dependent protein kinase as well as a coding gene and an application thereof. The sequences of the gene and the protein are shown as SED ID NO.1 and SED ID NO.2 in a gene coding sequence table respectively. Based on physiological and biochemical detection of the mulberry under the cadmium stress and the disclosure of the mulberry calcium dependent protein kinase MmCDPK2, the physiological and biochemical detection of the mulberry under the cadmium stress and the expression analysis and application of the signal channel protein are improved. The mulberrycalcium dependent protein kinase can be used for physiological and biochemical change analysis of the mulberries under cadmium stress and gene research of signal channel protein in the mulberries. Byusing a VIGS technology, the mulberry calcium dependent protein kinase MmCDPK2 gene is transferred into agrobacterium tumefaciens GV3101, and the gene expression is reduced by using a virus silencingprinciple; and physiologic