PCR/LDR molecular marker and method for identifying rice fragrance allele Badh2-E7

• Main Authors: CAO LIMING, TU RONGJIAN, CHENG CAN, NIU FU'AN, CHU HUANGWEI, ZHOU JIHUA, SUN BIN
• Format: Patent
• Language: Chinese; English
• Published: 17.07.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a PCR/LDR molecular marker and method for identifying a rice fragrance allele Badh2-E7, and belongs to the technical field of rice breeding. The molecular marker comprises a pair of PCR primers and three LDR probes, wherein the forward primer sequence of the PCR primer pair is shown as SEQ ID NO.1, and the reverse primer sequence is shown as SEQ ID NO.2. The LDR probe comprises a fluorescent labeled probe Probe-FAM with a sequence as shown in SEQ ID NO.3, a non-Badh2-E7 allele specific probe Probe-non with a sequence as shown in SEQ ID NO.4, and a Badh2-E7 allele specific probe Probe-E7 with a sequence as shown in SEQ ID NO.5. The method not only can accurately and rapidly identify whether strains in a rice variety or a breeding population carry the Badh2-E7 alleleor not, but also can realize high-throughput detection of many samples. 本发明涉及一种鉴定水稻香味等位基因Badh2-E7的PCR/LDR分子标记和方法，属于水稻育种技术领域。本发明所述分子标记包括一对PCR引物和三条LDR探针，PCR引物对正向引物序列如SEQ ID NO.1所示，反向引物序列如SEQ ID NO.2所示。LDR探针包括一条荧光标记探针Probe-FAM，