Preparation method of fermented type Evernia extract for cigarettes

• Main Authors: CUI HONGLIANG, LIU WEIJUAN, WU PINGYAN, WEI QING, CAO HUAN, ZHANG SHIDONG
• Format: Patent
• Language: Chinese; English
• Published: 26.06.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; CIGARETTES; CIGARS; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING ORCHEWING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SMOKERS' REQUISITES; SNUFF; SPIRITS; TOBACCO; VINEGAR; WINE

The invention discloses a preparation method of a fermented type Evernia extract for cigarettes. The preparation method comprises the following steps of: firstly, carrying out activated culture on anAspergillus niger strain RSZZ-6-1 to obtain an Aspergillus niger activated strain; then, respectively crushing dried Evernia and tobacco stems, then adding water with the volume being 3-5 times that of the crushed Evernia and tobacco stems, and carrying out sterilizing to obtain a mixture; inoculating the Aspergillus niger activated strain into the mixture for fermentation culture; performing extracting treatment on an obtained fermented product by using ethanol; carrying out centrifugal treatment on an extracting solution, and concentrating a supernatant into an extractum; and adding propylene glycol with the mass ratio of 2-20% of the concentrated extractum into the concentrated extractum to obtain the required Evernia extract. The Evernia extract prepared by the preparation method disclosed by the invention can