Double-layer artificial skin culture device and use method thereof

The invention discloses a double-layer artificial skin culture device and a use method thereof. The device comprises a culture box and a sealing cover, wherein the culture box is internally provided with a telescopic rod and a culture stand; one end of the telescopic rod is mounted on the bottom pla...

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Bibliographic Details
Main Authors XING ZHIQING, LI FEN, ZHANG PING, ZHANG TIANTIAN, SHAN FENGJUAN
Format Patent
LanguageChinese
English
Published 26.06.2020
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a double-layer artificial skin culture device and a use method thereof. The device comprises a culture box and a sealing cover, wherein the culture box is internally provided with a telescopic rod and a culture stand; one end of the telescopic rod is mounted on the bottom plate of the culture box; and the other end of the telescopic rod is mounted on the culture stand. Theuse method of the double-layer artificial skin culture device comprises the following steps: compressing the pressing rod to enable the culture stand to fit to the bottom plate of the culture box; placing a layer of a nylon membrane on the culture stand; adding a layer of a cell-free collagen on the nylon membrane; inoculating dermal fibroblasts, adding a culture liquid, covering the components bya sealing cover, and performing culture so as to form a dermis layer; opening the sealing cover, inoculating epidermal cells on the dermis layer, covering the components by the sealing cover, and culturing the epidermal cells
Bibliography:Application Number: CN202010227437