Protein immunoblotting detection method for improving antibody specificity of rabbit antiserum

• Main Authors: ZHANG FANG, SHU QIN, YIN TINGZI, YI WANGXUE, HUA QUANGAO, ZHANG XUEJIAO, ZHAO YUAN'AN
• Format: Patent
• Language: Chinese; English
• Published: 23.06.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a protein immunoblotting detection method for improving antibody specificity of rabbit antiserum. The protein immunoblotting detection method comprises the following steps: preparing a positive to-be-detected sample containing target protein; carrying out electrophoretic separation on the positive to-be-detected sample according to the size of protein to obtain separated to-be-detected protein with a specific size; preparing an antigen solid-phase carrier carrying the protein to be detected; soaking the antigen solid-phase carrier in a confining liquid to obtain a confined antigen solid-phase carrier; incubating and combining the sealed antigen solid-phase carrier with rabbit antiserum to obtain an incubated antigen-antibody compound solid-phase carrier; incubating and combining the antigen-antibody complex solid-phase carrier with a secondary antibody to obtain a solid-phase carrier of an antigen-antibody enzyme-labeled complex; and then carrying out enzyme chromogenic detection by usi