Recombinant staphylococcal protein A as well as preparation method and application thereof

• Main Authors: CHANG GUISHENG, TANG XIANGZHONG, ZHU WENJIN, XIE LEI, ZHANG CHAO
• Format: Patent
• Language: Chinese; English
• Published: 23.06.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a recombinant staphylococcal protein A as well as a preparation method and an application thereof, belonging to the field of biotechnology. The recombinant staphylococcal protein A is a recombinant protein obtained by adding a short peptide YJ at the N end of natural staphylococcal protein A; and the amino acid sequence of the short peptide YJ is as shown in SEQ ID NO. 1.The invention also provides a recombinant staphylococcal protein A gene, which is named as yj-rspa gene; and the nucleotide sequence of the gene is shown as SEQ ID NO. 2. Compared with the prior art,the expression quantity of the optimized gene in a prokaryotic expression system is remarkably improved, and an important significance to the industrial production of YJ-rSPA and the application to the field of biological medicine field is achieved. 本发明涉及一种重组金黄色葡萄球菌A蛋白及其制备方法与应用，属于生物技术领域。重组金黄色葡萄球菌A蛋白即在天然金黄色葡萄球菌A蛋白的N端加了一个短肽YJ后得到的重组蛋白，所述短肽YJ氨基酸序列如SEQ ID NO.1所示。本发明还提供一种重组金黄色葡萄球菌A蛋白基因，命名为yj-rspa基因，所述基因的核苷酸序列如SEQ ID NO.2所示。与现有技术