Enzyme-loaded calcium alginate microsphere enhanced cascade enzymatic reaction method based on aqueous two-phase system

• Main Authors: SUN HEJIA, LIU GANG, LIU TIANTIAN, WANG YAOLEI, MENG TAO, JIN LINQI
• Format: Patent
• Language: Chinese; English
• Published: 19.06.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an enzyme-loaded calcium alginate microsphere enhanced cascade enzymatic reaction method based on an aqueous two-phase system. The method comprises the following steps: step 1,respectively preparing a PEG aqueous solution with a mass concentration of 8% and a Dex aqueous solution with a mass concentration of 8%, and performing full mixing and phase splitting, wherein the upper phase is a PEG solution and the lower phase is a Dex solution; step 2, adding sodium alginate and a target enzyme solution into the Dex solution to form a Dex-sodium alginate solution containinga target enzyme, wherein the mass concentration of the sodium alginate is 0.5%; step 3, taking the PEG solution prepared in the step 1 as an outer water phase and the Dex-sodium alginate solution containing the target enzyme obtained in the step 2 as an inner water phase, and forming aqueous two-phase sodium alginate droplets at the conical tip through a coaxial capillary tube; and step 4, introducing the aqueous two-phase