Isolated culture method of free pluripotent stem cells in sheep tissues and culture solution

The invention provides an isolated culture method of free pluripotent stem cells in sheep tissues and a culture solution, and belongs to the technical field of cell culture. The culture solution takesDMEM as a basic culture solution, and further comprises L-glutamine, non-essential amino acids, sodi...

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Main Authors ZHANG QIONGLIN, GENG WANHENG, JIN HAI, YUN FUYU, LI XIAOQI, WANG FENGWU
Format Patent
LanguageChinese
English
Published 19.06.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides an isolated culture method of free pluripotent stem cells in sheep tissues and a culture solution, and belongs to the technical field of cell culture. The culture solution takesDMEM as a basic culture solution, and further comprises L-glutamine, non-essential amino acids, sodium pyruvate, beta-mercaptoethanol, LIF, bFGF and FBS. The isolated culture method comprises the following steps: cleaning sheep germ layer tissue blocks, placing the cleaned sheep germ layer tissue blocks in a culture solution, needling the tissue blocks and extruding, collecting the culture solution, centrifuging, collecting supernatant, centrifuging again, collecting precipitates, re-suspending cells, and culturing. The separated pluripotent stem cells can stably express marker genes relatedto the characteristics of the pluripotent stem cells, and do not express or weakly express Cx40 and Cx43 at the same time. The establishment of the method is of great significance to further deep research of stem cells, organ
Bibliography:Application Number: CN201911167203