Method for producing coenzyme Q10 by combining plasma action with oxygen limitation

• Main Authors: ZHUANG YINGPING, HANG HAIFENG, GUO MEIJIN, ZHANG XINGZI, ZHU ZHICHUN, CHEN BIQIN, ZHAN GUANGHUANG, WANG ZEJIAN, LI DAN
• Format: Patent
• Language: Chinese; English
• Published: 16.06.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for producing coenzyme Q10 by combining plasma action with oxygen limitation, which comprises the following steps: uniformly covering the surface of a slide with a bacterial suspension, putting the slide in an ARTP mutagenesis instrument, and carrying out mutagenesis treatment and elution to obtain the mutagenized bacterial suspension; diluting the mutated bacterial suspension under an aseptic condition, coating the diluted bacterial suspension on a flat plate containing a flat plate culture medium added with anhydrous sodium sulfite, and culturing to obtain aflat plate with a single colony; picking single colonies from the flat plate with the single colonies, putting the single colonies into a 24-pore plate, adding a seed culture medium, covering the 24-pore plate with a cover, and culturing to obtain a seed solution; and fermenting to produce the coenzyme Q10. The rhodobacter sphaeroides production strain with improved specific growth rate, oxygen affinity and product synthe