SIZE SELECTION PURIFICATION USING THERMOPLASTIC SILICA NANOMATERIAL

• Main Authors: LIU KELVIN JENG-FANG, BURKE JEFFREY MICHAEL, KILBURN JOHN DUNCAN
• Format: Patent
• Language: Chinese; English
• Published: 12.06.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR RELEVANT APPARATUS; TRANSPORTING; VINEGAR; WINE

The present disclosure is directed to a method for purifying a sample containing nucleic acids to obtain isolated nucleic acids of a desired size range, either above a size cut-off, below a cut-off, or within a defined band of sizes, including: a) combining a nucleic acid-containing sample with a binding buffer to provide a binding mixture; b) contacting the binding mixture with a silica nanomembrane, wherein the silica nanomembrane adsorbs nucleic acids from the binding mixture within a desired size-range; and c) separating the bound nucleic acid from the remaining sample. Kits including a silica nanomembrane, a binding buffer and one or wash buffers are also provided herein. 本公开涉及一种用于纯化含核酸的样品以获取分离的所需尺寸范围的核酸的方法，所述所需尺寸范围可以是大于尺寸截留值、小于尺寸截留值、或在限定的尺寸带内，所述方法包括：a)将含核酸的样品与结合缓冲液混合以提供结合混合物；b)使结合混合物与硅石纳米膜接触，其中该硅石纳米膜从结合混合物中吸附在所需尺寸范围内的核酸；以及c)从其余样品中分离结合的核酸。本文还提供了包括硅石纳米膜、结合缓冲液和一种或洗涤缓冲液的试剂盒。