Construction method of recombinant clostridium acetobutylicum and application of recombinant clostridium acetobutylicum in preparation of butanol by fermenting hemicellulose

• Main Authors: LIU DONG, YING HANJIE, WANG ZHENYU, CHEN YONG, LI NAN, CAO XINGYUAN, YANG ZHENGJIAO, ZHU CHENJIE
• Format: Patent
• Language: Chinese; English
• Published: 12.06.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a construction method of recombinant clostridium acetobutylicum and application of the recombinant clostridium acetobutylicum in preparation of butanol by fermenting hemicellulose. The recombinant clostridium acetobutylicum is constructed by overexpression of xylanase genes in clostridium acetobutylicum. Compared with the prior art, the invention has the following advantages: (1) the xylanase gene is overexpressed in clostridium acetobutylicum to successfully express the xylanase, and the xylanase has high solubility and can be completely secreted out of cells, therebyeffectively enhancing the hemicellulose degradation capacity of clostridium acetobutylicum; (2) the biological process of preparing butanol by fermentation by taking hemicellulose as a unique carbon source is realized, the cost of preparing butanol is greatly reduced, and the feasibility of converting renewable biomass energy into biofuel is further verified; and (3) compared with wild bacteria, the recombinant clostridiu