Method for creating rice germplasm with high grain weight, low chalkiness, medium amylose content and low gelatinization temperature

• Main Authors: LI AIHONG, HUANG NIANSHENG, DAI ZHENGYUAN, PAN CUNHONG, JI HONGJUAN, YU LING, LI YUHONG, WU YUNYU, LIU GUANGQING, ZHANG XIAOXIANG, XIAO NING, WANG ZHIPING, ZHOU CHANGHAI, CAI YUE
• Format: Patent
• Language: Chinese; English
• Published: 09.06.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a method for creating rice germplasm with high grain weight, low chalkiness, medium amylose content and low gelatinization temperature. According to the method, key SNP variation sites for rice grain weight, grain length genes (gs3, GW5, GL7 and Lgy3), amylose content genes (Wx) and gelatinization temperature genes (ALK) are clarified, a set of KASP markers capable of identifying different grain types, amylose content and gelatinization temperature is developed, the developed KASP markers are used for carrying out genotype identification on existing varieties, on the basis of genotype classification, favorable genotype varieties with long grains, high grain weight, medium amylose content and low gelatinization temperature are selected for hybridization and backcross, and a germplasm resource with high grain weight, low chalkiness rate and low gelatinization temperature are directionally selected and created. 本发明公布了一种创制高粒重、低垩白、中等直链淀粉含量和低糊化温度水稻种质的方法。本发明明确了水稻粒重、粒长基因(gs3、GW5、GL7以及Lgy3)、直链淀