Method for determining L-carnosine through efficient capillary electrophoresis and application of L-carnosine in quality evaluation of polaprezinc

• Main Authors: HUANG XI, ZHENG CHENGYUN, CHEN YISHAN, XIAO FUYAN, LI JIE, FAN JUNXIA, QI XUEYONG, FANG QIUYU
• Format: Patent
• Language: Chinese; English
• Published: 02.06.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention belongs to the technical field of analytical chemistry, and relates to a method for determining L-carnosine through efficient capillary electrophoresis, which comprises the following steps: (1) dissolving L-carnosine in a buffer solution to prepare reference substance stock solutions, respectively measuring a plurality of reference substance stock solutions in a 10 mL measuring flask, fixing the volume to obtain a series of concentration reference substance solutions, and storing at 4 DEG C; (2) performing sample injection detection on the series of concentration reference substance solutions by adopting high performance capillary electrophoresis, recording a chromatogram, and drawing a standard curve by taking the peak area A of an analyte as a vertical coordinate and the concentration C of the reference substance solution as a horizontal coordinate; and (3) pretreating a sample to be detected, measuring the peak area through high performance capillary electrophoresis, and measuring the content