一种检测肠道菌群代谢气体的呼气试验方法

• Format: Patent
• Language: Chinese
• Published: 05.11.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

本发明提供一种检测肠道菌群代谢气体的呼气试验方法，受试者空腹12小时后首先测试空腹的呼气H2、CH4与H2S的浓度，然后服用可通过菌群代谢产生H2、CH4与H2S的底物，在服用底物1-3小时内每20-30分钟测试一次呼气的H2、CH4与H2S浓度，根据服用底物前后的呼气浓度对肠道菌群代谢相关的疾病进行诊断。所述底物含葡萄糖或乳果糖、乳酸盐或苹果酸盐与硫酸氨基葡萄糖或硫酸锌，三种成分的重量比范围为10：1：2~10：1：0.5。本发明方法采用的检测装置为一种可同时检测呼气H2、CH4、H2S与CO2的装置，H2与CH4的检测限与精度为1-2ppm,H2S的检测限与精度为1-2ppb，呼气时动态监测鼻呼气CO2并在CO2达到平台期后采样分析鼻呼气的H2、CH4、H2S。 The invention provides an expiration test method for detecting intestinal flora metabolic gas. The method comprises the following steps: 12 hours after a subject has an empty stomach, firstly testingthe concentrations of H2, CH4 and H2S exhaled by the empty stomach; and then taking a substrate capable of generating H2, CH4 and H2S through flora metabolism, testing the concentrations of H2, CH4 and H2S of expiration every 20-30 minutes within 1-3 hours after taking the substrate, and diagnosing diseases related to intestinal flora metabolism according to the concentrations of H2, CH4 and H2S before and after taking the substrate, wherein the substrate contains glucose or lactulo