Yeast engineered strain with tolerance to inhibitors including furfural and so on and constructing method for yeast engineered strain and application of yeast engineered strain

• Main Authors: XIAO DIFAN, ZHAO XIANXIAN, OUYANG YIDAN, MA MENGGEN, KUANG XIAOLIN, WANG HANYU, LI QIAN
• Format: Patent
• Language: Chinese; English
• Published: 05.05.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a yeast engineered strain with tolerance to inhibitors including furfural and so on and a constructing method for the yeast engineered strain and application of the yeast engineered strain. The yeast engineered strain with the tolerance to the inhibitors including the furfural and so on is named as PYR, and is classified and named as saccharomyces cerevisiae, and the yeastengineered strain is collected in China General Microbiological Culture Collection Center on June 14, 2019, and the collection number is CGMCC No.17930. The engineered strain can be applied to preparing second generation biofuel ethanol and other bio-based chemicals taking a lignocellulose hydrolysate as a raw material; and according to the constructing method, the yeast engineered strain is constructed by uniting a transcriptional regulation gene with overexpression, and thus, a technological means is provided for reforming yeast strains from the gene expression level. 本发明公开了一种糠醛等抑制物耐受酵母工程菌株及其构建方法与应用，该糠醛等抑制物耐受酵母工程菌，命