METHODS FOR DETECTING Na/K-ATPASE-MEDIATED SRC SIGNALING FOR DIAGNOSIS AND PROGNOSIS OF CANCER

• Main Authors: CUI XIAOYU, XIE ZIJIAN, BANERJEE MOUMITA
• Format: Patent
• Language: Chinese; English
• Published: 01.05.2020
• Subjects: CHEMISTRY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; TESTING

Methods for diagnosis or prognosis of a cancer in a subject are provided and include the steps of: obtaining a biological sample from a subject; determining an amount of a phosphorylation at a Y260 residue in a Na/K ATPase present in the biological sample; and comparing the amount of the phosphorylation in the sample to a control level to thereby diagnose the cancer. Methods for detecting a metabolic switch from oxidative phosphorylation to aerobic glycolysis are also provided in which a biological sample including one or more cells is obtained and an amount of a phosphorylation at a Y260 residue in a Na/K ATPase is determined in the one or more cells. 提供了用于对象中癌症的诊断或预后的方法，所述方法包括下述步骤：从对象获得生物样品；确定所述生物样品中存在的Na/K ATP酶中Y260残基处磷酸化的量；以及将所述样品中所述磷酸化的量与对照水平进行比较，从而诊断所述癌症。还提供了用于检测从氧化磷酸化向有氧糖酵解的代谢切换的方法，其中获得包括一个或多个细胞的生物样品，并在所述一个或多个细胞中确定Na/K ATP酶中Y260残基处磷酸化的量。