Preparation method and application of decellularized fishskin substrates

• Main Authors: SONG GUOLIANG, XIE ZEPING, LI DONGSHENG, LI CHENGBO, GONG SHIZHOU, QU CHENGLEI, SHEN SHENGBIAO, ZHANG SHUMIN
• Format: Patent
• Language: Chinese; English
• Published: 01.05.2020
• Subjects: CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; HUMAN NECESSITIES; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL

The invention discloses a preparation method of novel decellularized fishskin substrates. The method comprises the following steps of (1) performing pretreatment of washing Tilapia skin, removing impurities and the like; (2) after acid treatment, scraping scales; (3) performing washing with a surfactant for decellularization; (4) performing washing with alkali liquor; (5) performing treatment witha chemical bleaching agent; and (6) performing post-treatment of drying, sterilization and the like. The decellularized fishskin substrates prepared by the preparation method disclosed by the invention have the advantages of being low in production cost, high in heat stability, free from zoonoses virus propagation risk and the like, and besides, a scale removing technique is also innovatively provided, so that the clinical practicality of products is greatly increased; and various techniques are comprehensively applied for virus inactivation, so that the biological safety of the products is increased. Besides, the in