Insulin determination kit capable of eliminating interference of insulin autoantibody

• Main Authors: MIAO ZHENWEI, LI MINGWEI, LI LIHE, SUN GUOZHONG, CUI ZHE, DING HONG
• Format: Patent
• Language: Chinese; English
• Published: 28.04.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses an insulin determination kit for eliminating the interference of an insulin autoantibody, and belongs to the technical field of immunoassay. The technical scheme of the invention is as follows: a sample diluent contains an insulin autoantibody secondary antibody, the insulin autoantibody secondary antibody in the sample diluent is combined with the insulin autoantibody in the serum, and the competitive reaction between the insulin autoantibody and the insulin antibody and the insulin in an insulin determination reagent is eliminated. The insulin detection is not affected by the insulin autoantibody, the use method is the same as the original chemiluminescence method, and the method is economical, convenient and practicable and is an insulin detection method with high accuracy. 本发明公开了一种消除胰岛素自身抗体干扰的胰岛素测定试剂盒，属于免疫分析技术领域。本发明的技术方案是：在样品稀释液中含有胰岛素自身抗体二抗，样品稀释液中胰岛素自身抗体二抗与血清中胰岛素自身抗体结合，消除了胰岛素自身抗体与胰岛素测定试剂中胰岛素抗体和胰岛素产生的竞争性反应，本发明检测胰岛素时不受胰岛素自身抗体影响，其使用方法与原有化学发光法相同，经济方便易行，是一种准确性更高的胰岛素检测方法。