Engineering bacteria genetically modified by neomycin biosynthetic gene cluster, and applications thereof

• Main Authors: LI YUE, ZHENG JIAZHEN, TAN HUARONG, ZHANG JIHUI, GUAN HANYE, TIAN YUQING
• Format: Patent
• Language: Chinese; English
• Published: 17.04.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a method for constructing a high-yield neomycin engineering strain streptomyces fradiae. The method comprises: introducing a neomycin biosynthetic gene cluster of streptomyces fradiae into wild streptomyces fradiae. The method can also comprise: carrying out genetic engineerin...