Engineering bacteria genetically modified by neomycin biosynthetic gene cluster, and applications thereof
The invention provides a method for constructing a high-yield neomycin engineering strain streptomyces fradiae. The method comprises: introducing a neomycin biosynthetic gene cluster of streptomyces fradiae into wild streptomyces fradiae. The method can also comprise: carrying out genetic engineerin...
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Main Authors | , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
17.04.2020
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Subjects | |
Online Access | Get full text |
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Summary: | The invention provides a method for constructing a high-yield neomycin engineering strain streptomyces fradiae. The method comprises: introducing a neomycin biosynthetic gene cluster of streptomyces fradiae into wild streptomyces fradiae. The method can also comprise: carrying out genetic engineering modification on the neomycin biosynthetic gene cluster of streptomyces fradiae to be introduced into wild streptomyces fradiae, wherein genetic engineering modification can comprise: blocking the negative regulatory gene neoI in the neomycin biosynthetic gene cluster or blocking the negative regulatory gene neoI in the neomycin biosynthetic gene cluster while driving the transcription of a key enzyme encoding gene neoE-neoD co-transcription unit by using a strong promoter. The invention also provides high-yield neomycin streptomyces fradiae obtained by the method.
本发明提供构建新霉素高产工程菌株弗氏链霉菌(Streptomyces fradiae)的方法,所述方法包括将弗氏链霉菌的新霉素生物合成基因簇导入野生型弗氏链霉菌中。所述方法还可以包括对要导入野生型弗氏链霉菌中的弗氏链霉菌的新霉素生物合成基因簇进行基因工程改造,所述基因工程改造可以包括对新霉素生物合成基 |
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Bibliography: | Application Number: CN201811176264 |