Free thyroxine luminescence immunoassay kit and preparation method thereof

• Main Authors: HAN MEIYU, WANG XIANGQI, HE HAOHUI, KAN HONGJING, LIU ZILING, LIU TINGTING, CONG FANHAO
• Format: Patent
• Language: Chinese; English
• Published: 10.04.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a free thyroxine luminescence immunoassay kit and a preparation method thereof, and belongs to the technical field of immunoassay. The kit comprises the following reagents: a solid-phase reagent R1: a suspension containing streptavidin magnetic particles; a liquid phase reagent R2: a suspension containing an acridinium ester labeled T4 antibody; a biotin reagent R3: a suspension containing a biotin-labeled thyroxine derivative. The streptavidin-containing magnetic particles in the R1 are ferroferric oxide of which the surface is coated with streptavidin. The invention also provides a preparation method of the free thyroxine luminescence immunoassay kit. Free thyroxine in serum/plasma is quantitatively detected by adopting a chemiluminescence immunoassay method, and the kit has the advantages of being rapid in detection, wide in linear range, high in sensitivity, few in interference factor, low in cost and the like. 本发明提供一种游离甲状腺素发光免疫检测试剂盒及其制备方法，属于免疫分析技术领域。该试剂盒包括以下试剂：固相试剂R1：含有链霉亲和素磁颗粒的悬液；