Treatment method for promoting germination of curculigo orchioides seeds
The invention discloses a treatment method for promoting germination of curculigo orchioides seeds. The treatment method comprises the steps of collecting maturing curculigo orchioides seeds, removingseed coats, performing soaking in gibberellin and performing germ-free germination, wherein the conc...
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Main Authors | , , , |
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Format | Patent |
Language | Chinese English |
Published |
27.03.2020
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a treatment method for promoting germination of curculigo orchioides seeds. The treatment method comprises the steps of collecting maturing curculigo orchioides seeds, removingseed coats, performing soaking in gibberellin and performing germ-free germination, wherein the concentration of the gibberellin for soaking is 100mg/L, the soaking time is 24h, and the germ-free germination is performed on a superclean bench; performing sterilization treatment on the seeds treated with the gibberellin, inoculating the sterilized seeds with culture mediums, and performing cultureunder the appropriate culture condition, wherein the sterilization treatment is performed through the steps of sterilizing seeds with 75% ethanol for 30s, performing flushing with sterile water twice, then performing sterilization with 0.1% mercury dichloride for 8min, and performing flushing with sterile water for 5 times, the appropriate culture condition refers to the condition of 25 DEG C, 2500Lx and 12 h/d, and the c |
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Bibliography: | Application Number: CN201911211232 |