SNP analysis technique based on NGS, for detecting brain gliomas 1p and 19q chromosomes

• Main Author: CHEN YONGWU
• Format: Patent
• Language: Chinese; English
• Published: 24.03.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention provides an SNP analysis technique based on NGS, for detecting brain gliomas 1p and 19q chromosomes. The technique comprises the following steps of step (1), extracting DNA of a sample to be detected, and performing quantitative detection on the concentration by Qubit 3.0, wherein the sample with the qualified concentration is used as a high-flux sequencing formwork; step (2) after genomic DNA is interrupted, performing end repair; step (3), purifying connector connection and connector connection products; step (4), after a pre-library is amplified, purifying the amplified pre-library; step (5), performing hybridization on the pre-library; and step (6), performing catching, eluting, preparing an end library and purifying the end library. According to the SNP analysis technique method based on NGS, 1p and/or 19q deletion can also be reliably detected in background tumor tissue of 70% of normal cells, and the method is more sensitive than LOH analysis based on microsatellite. Less DNA is needed, a