High-throughput sequencing method of macrobenthos COI genes and application of high-throughput sequencing method of macrobenthos COI genes

• Main Authors: DING SEN, JIA XIAOBO, SUN WEI, REN YIXUAN, CAO SHENGJIE, ZHANG YUAN, WANG SHUPING, XIA RUI, LIN JIANING, WANG HUI
• Format: Patent
• Language: Chinese; English
• Published: 20.03.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of macrobenthos identification, and particularly relates to a high-throughput sequencing method of macrobenthos COI genes and application of the high-throughput sequencing method of the macrobenthos COI genes. The high-throughput sequencing method of the macrobenthos COI genes comprises the following steps: (1) with DNA samples of mixed macrobenthos samples as templates, conducting PCR amplification on the COI genes; (2) with PCR amplification products in the step (1) as templates, conducting PCR amplification to obtain PCR products as DNA libraries, wherein an amplification primer pair comprises pyrophosphoric acid sequencing high-throughput sequencing connectors, and each sample corresponds to one pyrophosphoric acid sequencing high-throughputsequencing connector; and (3) conducting quality detection on the obtained DNA libraries, and then choosing qualified DNA libraries for emPCR amplification and high-throughput sequencing. According to the method, through one