High-throughput sequencing method of macrobenthos COI genes and application of high-throughput sequencing method of macrobenthos COI genes
The invention belongs to the technical field of macrobenthos identification, and particularly relates to a high-throughput sequencing method of macrobenthos COI genes and application of the high-throughput sequencing method of the macrobenthos COI genes. The high-throughput sequencing method of the...
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Main Authors | , , , , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
20.03.2020
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Subjects | |
Online Access | Get full text |
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Summary: | The invention belongs to the technical field of macrobenthos identification, and particularly relates to a high-throughput sequencing method of macrobenthos COI genes and application of the high-throughput sequencing method of the macrobenthos COI genes. The high-throughput sequencing method of the macrobenthos COI genes comprises the following steps: (1) with DNA samples of mixed macrobenthos samples as templates, conducting PCR amplification on the COI genes; (2) with PCR amplification products in the step (1) as templates, conducting PCR amplification to obtain PCR products as DNA libraries, wherein an amplification primer pair comprises pyrophosphoric acid sequencing high-throughput sequencing connectors, and each sample corresponds to one pyrophosphoric acid sequencing high-throughputsequencing connector; and (3) conducting quality detection on the obtained DNA libraries, and then choosing qualified DNA libraries for emPCR amplification and high-throughput sequencing. According to the method, through one |
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Bibliography: | Application Number: CN201910840869 |