Screening method for bacteria for degrading atrazine and cellulose

The invention relates to the technical field of isolation of microbes, in particular to a screening method for bacteria for degrading atrazine and cellulose. The screening method for the bacteria fordegrading the atrazine and the cellulose comprises the steps of (1) taking forest soil covered with v...

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Bibliographic Details
Main Authors WANG CHONGJU, GONG CHUNJIE
Format Patent
LanguageChinese
English
Published 20.03.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to the technical field of isolation of microbes, in particular to a screening method for bacteria for degrading atrazine and cellulose. The screening method for the bacteria fordegrading the atrazine and the cellulose comprises the steps of (1) taking forest soil covered with vegetation, straw and fallen leaves all year around; inoculating the forest soil onto an enrichmentmedium by employing a concentration gradient method, culturing the inoculated forest soil until single colonies grow out, transferring the single colonies onto a plate taking carboxymethyl cellulose as a unique carbon source for purification until single colonies grow out; (2) performing continuous passage culture on the purified single colonies on the plate taking the carboxymethyl cellulose as the unique carbon source; (3) transferring the single colonies onto a plate taking microcrystalline cellulose powder as a unique carbon source until single colonies grow out, and performing continuouspassage culture; (4) transf
Bibliography:Application Number: CN201911220195