Glucose oxidase M5GOD and coding gene and application thereof

• Main Authors: MOU HAIJIN, YUAN MINGXUE, YU DONGXING, YU WANSHUAI, LIU ZHEMIN
• Format: Patent
• Language: Chinese; English
• Published: 17.03.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT,VEGETABLES, EDIBLE SEEDS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THE PRESERVED, RIPENED, OR CANNED PRODUCTS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The invention discloses glucose oxidase M5GOD and a coding gene and application thereof, and relates to the field of gene engineering and fermentation engineering. The amino acid sequence of the glucose oxidase provided by the invention is shown as SEQ ID NO.2. The invention further provides a nucleotide sequence of a coding gene for coding the glucose oxidase, which is shown as SEQ ID NO.4. The invention further discloses a recombinant vector and a recombinant strain containing the gene and the application thereof. According to the invention, the glucose oxidase has good properties; the optimum pH value is 5.5; the optimum temperature is 30 DEG C; the glucose oxidase has good low temperature adaptability; the glucose oxidase serves as a novel enzyme preparation, and can be widely appliedto the industries of feed, food, medicines and the like. 本发明公开了一种葡萄糖氧化酶M5GOD及其编码基因和应用，涉及基因工程及发酵工程领域。本发明提供的一种葡萄糖氧化酶的氨基酸序列如SEQ ID NO.2所示，且本发明还提供了编码上述葡萄糖氧化酶的编码基因的核苷酸序列如SEQ ID NO.4所示。包含该基因的重组载体和重组菌株及其应用。本发明的葡萄糖氧化酶具有良好的性质，最适pH 5.5