DNA bar codes for identifying Thryssa mystax and Thryssa kammalensis, and identification method and application thereof

• Main Authors: WANG HAISHAN, CHEN ZHI, YE LE, YANG CHAOJIE, SU LICAN, XING XIAOXUAN
• Format: Patent
• Language: Chinese; English
• Published: 13.03.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides DNA bar codes for identifying Thryssa mystax and Thryssa kammalensis, and an identification method and application thereof, belonging to the technical field of molecular biology. The sequence of the DNA bar code for Thryssa mystax is as shown in SEQ ID No. 1; the sequence of the DNA bar code for Thryssa kammalensis is as shown in SEQ ID No. 2. Primers for amplifying the DNAbar codes comprise a primer F295 and a primer R517. The method comprises the following steps: extracting the genome DNA of a sample to be identified; amplifying the DNA to be identified by using the primers; comparing the sequences of amplification products with the DNA bar codes; and determining the type of the to-be-identified sample. When the DNA bar codes provided by the invention are used foridentifying Thryssa mystax and Thryssa kammalensis, results are stable, and repeatability is strong. 本发明提供了一种鉴别中颌棱鳀与赤鼻棱鳀的DNA条形码及其鉴别方法和应用，属于分子生物学技术领域，本发明所述中颌棱鳀的DNA条形码序列如SEQ ID No.1所示；所述赤鼻棱鳀的DNA条形码序列如SEQ ID No.2所示。扩增所述的DNA条形码的引