Stable recombinant cardiac troponin as well as coding gene and application thereof

• Main Authors: LIANG TING, JIN QIHANG, SHEN LIN, YU QIONGLIN, GE JUN, CHEN YUANCHAO, YANG BIN, XIAO CHANGHE
• Format: Patent
• Language: Chinese; English
• Published: 13.03.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides a stable recombinant cardiac troponin as well as a coding gene and an application thereof, which belong to the field of molecular biology. An amino acid sequence of the recombinant cardiac troponin is as shown in SEQ ID No. 1. The recombinant cardiac troponin provided by the invention has good stability, and the inactivation rate of the recombinant cardiac troponin placed at42 DEG C for one day is 12% and is significantly lower than that of conventional commercially available human serum-derived cTnI and cardiac troponin disclosed in the prior art. 本发明提供了一种稳定的重组心肌肌钙蛋白及其编码基因和应用，属于分子生物学领域。所述重组心肌肌钙蛋白的氨基酸序列如SEQ ID No.1所示。本发明提供的重组心肌肌钙蛋白具有良好的稳定性，在42℃条件下放置1天的失活率为12％，显著低于常规市售人源血清来源的cTnI和现有技术公开的心肌肌钙蛋白。