Application of KRT6A gene methylation in asthenospermia diagnostic agent and kit

• Main Authors: DU XINYA, XIE CHUN, WU BIN, DU YE, HUANG XIAOMING, TANG JIANXIN
• Format: Patent
• Language: Chinese; English
• Published: 07.02.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to application of the methylation level of a human KRT6A gene promoter region in preparation of a detection agent for detecting sperm motility. The human KRT6A gene promoter region is located in a region corresponding to a 52886681-52889381 site of a human No.12 chromosome. Analysis and research based on whole-genome methylation sequencing results and verification find that the methylation level of the human KRT6A gene promoter region has significant difference between asthenospermia patients and healthy people, and can be used as a molecular marker to diagnose whether subjects suffer from the asthenospermia. When the methylation rate of the region is higher than 0.72, the probability that the subjects suffer from the asthenospermia is larger. The molecular marker can provide auxiliary evaluation information for diagnosis of the asthenospermia and identify whether the subjects suffer from the asthenospermia or not in combination with other diagnosis indexes. 本发明涉及人KRT6A基因启动子区的甲基化水平在制备检测精