Quantum dot immunochromatographic test paper for quantitatively detecting estradiol in milk

• Main Authors: LU FEI, XIAO YOUYU
• Format: Patent
• Language: Chinese; English
• Published: 04.02.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses quantum dot immunochromatographic test paper for quantitatively detecting estradiol in milk. The test paper is prepared by sequentially adhering a sample pad, a marker pad, a chromatographic film and a buffer pad on a PVC (polyvinyl chloride) bottom plate in a manner of overlapping for 1-2mm, wherein the chromatographic film is a nitrocellulose film carrying a detection line and a quality control line; the detection line is coated with an estradiol antigen, and the quality control line is coated with a goat anti-mouse antibody; and the marker pad carries quantum dots coupled with the estradiol antibody. When the milk contains the estradiol, the estradiol flowing through the position of a T line can compete with the antigen coated by the T line to be combined with the antibody on the marker pad, so that the fluorescence intensity of detection is changed to perform quantitative detection. 本发明公开了一种定量检测牛奶中雌二醇的量子点免疫层析试纸，该试纸由样品垫、标记物垫、层析膜、缓冲垫相互重叠1-2mm依次粘贴在PVC底板上制得，所述层析膜为承载有检测线和质控线的硝酸纤维素膜，所述检测