Cas9-mediated dendrocalamus latiflorus gene editing vector and application thereof

• Main Authors: CHEN GANG, YE SHANWEN, ZHU QIANG
• Format: Patent
• Language: Chinese; English
• Published: 24.01.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The present invention discloses a Cas9-mediated dendrocalamus latiflorus gene editing vector and an application thereof. A Crispr/Cas9 dendrocalamus latiflorus expression vector is constructed, a Cas9gene is introduced into dendrocalamus latiflorus through an agrobacterium (EHA105) transformation method, a dendrocalamus latiflorus PSY1 gene is subjected to directional editing, and the method capable of editing the own gene of the dendrocalamus latiflorus to obtain a dendrocalamus latiflorus mutant is provided for the first time. Applicability of CRISPR/Cas9 in bamboo is proved, thereby promoting research and breeding of the bamboo in the future. 本发明公开了Cas9介导的麻竹基因编辑载体及其应用。发明通过构建Crispr/Cas9麻竹表达载体，通过农杆菌（EHA105）转化的方式，将Cas9基因导入麻竹中，对麻竹PSY1基因进行定向编辑，首次提供一种能够定向编辑麻竹自身基因从而获得麻竹突变体的方法。证明了CRISPR/Cas9在竹子中的适用性，从而促进了未来竹子的研究和育种。