Method for extracting anchorage-dependent cells and extracting device for method

• Main Authors: LIU ZHIHUI, LUO ZONGPING, SAIJILAFU, WANG JUEMING, GUO JIANGBO, CHEN XI
• Format: Patent
• Language: Chinese; English
• Published: 24.01.2020
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a method for extracting anchorage-dependent cells. The method comprises the following steps of preprocessing polyacrylamide hydrogel, culturing anchorage-dependent cells, integrally excavating the cells and the adhered gel, removing surface gel and the like. According to the method, an anchorage-dependent cell extracting device suitable for the extracting method is also arranged. The extracting method disclosed by the invention is convenient and easy to operate, and the extracting of the anchorage-dependent cells can be completed without tedious steps, so that the possibility of mistaking is reduced; besides, the adopted extracting device is simple in structure and low in cost; and in addition, the extracting method is small in damage to the anchorage-dependent cells, and has important significance to cell research. 本发明涉及一种贴壁细胞的提取方法，包括聚丙烯酰胺水凝胶预处理，贴壁细胞的培养，细胞及粘连凝胶的整体挖取以及去表面凝胶等步骤。同时本发明还设置一种适用于上述提取方法的贴壁细胞的提取装置。本发明提取方法操作方便易行，不需要繁琐的步骤便可以完成对于贴壁细胞的提取，减少了出错的可能性；同时采用的提取装置结构简单，成本低廉。另外本发明对于贴壁细胞的破