HPLC method for separating artabsin A from artabsin B

• Main Authors: ZHU YANGCHEN, ZHAO LING
• Format: Patent
• Language: Chinese; English
• Published: 17.01.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses an HPLC method for separating artabsin A from artabsin B. According to the method, a Waters Sunfire C18 liquid chromatographic column in the specification of 4.6 mm*250 mm and5 micron is adopted; the mobile phases include a phase A of a methanol aqueous solution in volume percentage concentration of 10% and with 10mM of a chiral additive and a phase B of a methanol aqueoussolution in the volume percentage concentration of 90% and with 10mM of a chiral additive; an elution process includes 0-5 min, 0% B; 5-20 min, 0%-80% of B; 20-25 min, 80%-0% of B; the flow velocityis 1.0mL/min; and the column temperature is 35 DEG C. According to the method, the chiral additive D-prolinamide is added into the mobile phase, so that the artabsin A can be separated from the artabsin B without the help of a chiral chromatographic column. Therefore, the HPLC method disclosed by the invention can be used for detecting whether the artabsin A is mixed with the artabsin B or whetherthe artabsin B is mixed wit