Primers and method for detecting related gene SNP (single nucleotide polymorphism) sites of metformin-individualized medications

• Main Authors: QI LINJIE, LI PENG, LENG HUAN
• Format: Patent
• Language: Chinese; English
• Published: 17.01.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to primers and a method for detecting related gene SNP (single nucleotide polymorphism) sites of metformin-individualized medications. The primers can detect a rs2289669SNP site of a SLC47A1 gene. The primer sequences comprise a wild-type upstream primer, a mutant-type upstream primer and a shared downstream primer. Improvement is made on the basis of ARMS-PCR, a specific primer and the shared downstream primer are designed for SNP sequences, mismatched bases are introduced at a second position of a 3' end of the specific primerto widen a gap between amplification efficiency of complementation and non-complementation of the bases of the 3' end of the specific primer and SNP site bases on a DNA template, thereby greatly improving specificity and accuracy of detection. A real-time PCR technology is introduced, identification can be made according to a [delta]Ct value, that is, the [delta]Ct value is equal to mutant Ct-wild Ct. In general, when [delta]Ct islarger than 7, the SNP sit