Mouse cytomegalovirus nucleic acid detection method

• Main Authors: HAN FEIFEI, JIA YANGJIE, LIU HE, AN ZHUOLING, LIU LIHONG, WAN ZIRUI, LYU YALI, GONG LILI, XUAN LINGLING
• Format: Patent
• Language: Chinese; English
• Published: 14.01.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a mouse cytomegalovirus nucleic acid detection method. The mouse cytomegalovirus nucleic acid detection method comprises the following steps: S1, constructing a standard plasmid; S2, constructing a detection standard curve; S3, extracting a DNA product to be tested from a sample to be tested, taking the DNA product to be tested as a template for carrying out PCR amplification so as to obtain DNA gene primer amplification product to be detected; performing fluorescence quantitative PCR on the DNA gene primer amplification product to be detected so as to obtain a CT value; obtaining concentration of the DNA gene primer amplification product to be detected according to the standard curve; and then, detecting mouse cytomegalovirus in the sample by performing qualitativeand quantitative detection. The mouse cytomegalovirus nucleic acid detection method has the following beneficial effects: the mouse cytomegalovirus nucleic acid detection method is capable of qualitatively detecting mouse cy