METHOD FOR INDUCING DIFFERENTIATION OF INTERMEDIATE MESODERMAL CELL TO RENAL PROGENITOR CELL, AND METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELL TO RENAL PROGENITOR CELL

• Main Authors: OSAFUNE KENJI, KASAHARA TOMOKO, SUETA SHINICHI
• Format: Patent
• Language: Chinese; English
• Published: 07.01.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

A method for producing renal progenitor cells from pluripotent stem cells, comprising steps (i) to (vi): (i) culturing the pluripotent stem cells in a culture medium containing FGF2, BMP4, a GSK-3[beta] inhibitor and retinoic acid or a derivative thereof; (ii) culturing cells produced in step (i) in a culture medium containing FGF2, a GSK-3[beta] inhibitor and BMP7; (iii) culturing cells producedin step (ii) in a culture medium containing FGF2, a GSK-3[beta] inhibitor, BMP7 and a TGF[beta] inhibitor; (iv) culturing cells produced in step (iii) in a culture medium containing FGF2, a GSK-3[beta] inhibitor, BMP7, activin and a ROCK (Rho-kinase) inhibitor; (v) culturing cells produced in step (iv) in a culture medium containing retinoic acid or a derivative thereof and FGF9; and (vi) culturing cells produced in step (v) in a culture medium containing a GSK-3[beta] inhibitor and FGF9 to induce renal progenitor cells from intermediate mesodermal cells. 一种由多能干细胞制造肾祖细胞的方法，包括以下的工序(i)～(vi)。(i)将多能干细胞用含有FGF2、BMP4、GSK-3β抑