METHOD FOR PRODUCING 2'-FUCOSYLLACTOSE BY USING FUCOSYLTRANSFERASE DERIVED FROM PSEUDOPEDOBATER SALTANS

• Main Authors: YOON JONG WON, SONG YOUNG HA, KIM BO MEE, YOU YOUNG SUN, KIM JIN KYUNG, LEE GA EUN, SHIN CHUL SOO
• Format: Patent
• Language: Chinese; English
• Published: 31.12.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a method for producing 2'-fucosyllactose in a recombinant Corynebacterium sp. microorganism into which Pseudopedobater saltans-derived fucosyltransferase is inserted.A recombinant Corynebacterium sp. microorganism, of the present invention, into which Pseudopedobater saltans-derived fucosyltransferase is inserted, can produce 2'-fucosyllactose in a high concentration, in a high yield and with high productivity, while being safer than conventional E. coli. 本发明涉及一种从插入源自舞蹈拟土地杆菌(Pseudopedobater saltans)的岩藻糖基转移酶(fucosyltransferase)的重组棒状杆菌属(Corynebacterium SP.)微生物中生产2'-岩藻糖基乳糖的方法，本发明的插入源自舞蹈拟土地杆菌(Pseudopedobater saltans)的岩藻糖基转移酶(fucosyltransferase)的重组棒状杆菌属(Corynebacterium SP.)微生物比常规大肠杆菌更安全，同时可以高浓度、高产率、高生产率生产2'-岩藻糖基乳糖。