MMLV REVERSE TRANSCRIPTASE VARIANTS

• Main Authors: MESCHI FRANCESCA, MCDERMOTT GEOFFREY, MONTESCLAROS LUZ, MARRS SAMUEL, ZIRALDO SOLONGO B, SHUGA JOSEPH FRANCIS, TERRY JESSICA MICHELE, LEE JOSEPHINE, PFEIFFER KATHERINE
• Format: Patent
• Language: Chinese; English
• Published: 31.12.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition,greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes. 本文公开了包含表现出若干所需性质诸如热稳定性、持续逆转录、非模板化碱基添加和模板转换能力的经工程改造逆转录酶的组合物、方法和试剂盒。相较于其他经工程改造逆转录酶，本文所述的经工程改造逆转录酶显示出乎意料的更高的对细胞溶解产物抑制的抗性、更大的捕集全长mRNA转录物的能力，并且在小反应体积的情况下显示出改进的结果。