Separation method of bacteria for degrading cellulose and petroleum hydrocarbon

The invention relates to the technical field of microbial separation, in particular to a separation method of bacteria for degrading cellulose and petroleum hydrocarbon. The separation method of bacteria for degrading cellulose and petroleum hydrocarbon comprises the following steps: (1) enrichment...

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Bibliographic Details
Main Authors WANG CHONGJU, GONG CHUNJIE
Format Patent
LanguageChinese
English
Published 31.12.2019
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to the technical field of microbial separation, in particular to a separation method of bacteria for degrading cellulose and petroleum hydrocarbon. The separation method of bacteria for degrading cellulose and petroleum hydrocarbon comprises the following steps: (1) enrichment culture: taking forest soil with grass, straw and fallen leaves falling throughout the year, inoculating the forest soil to an enrichment culture medium by adopting a concentration gradient method, performing culturing until a single colony appears, transferring the single colony to a flat plate taking carboxymethyl cellulose as a unique carbon source, and performing purifying until a single colony appears; (2) secondary screening: carrying out continuous passage on the single colony on a flat plate taking carboxymethyl cellulose as a unique carbon source; (3) directional screening: transferring the single colony to a flat plate taking microcrystalline cellulose powder as a unique carbon source until a single colon
Bibliography:Application Number: CN201910910605