Aptamer-imprinted monolithic column capable of specifically recognizing ochratoxin A and preparation method thereof

• Main Authors: LYU HAIXIA, XIE ZENGHONG, ZHU YIMEN
• Format: Patent
• Language: Chinese; English
• Published: 24.12.2019
• Subjects: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; HETEROCYCLIC COMPOUNDS; METALLURGY; ORGANIC CHEMISTRY; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; SEPARATION; THEIR RELEVANT APPARATUS; TRANSPORTING

The invention discloses a aptamer-imprinted monolithic column for specifically recognizing ochratoxin A and a preparation method thereof and belongs to the technical field of analytical chemistry. According to the monolithic column, highly hydrophilic 2-acrylamide-2-methylpropanesulfonic acid is used as an organic polymerization monomer, polyhedral oligomeric silsesquioxane and acrylate are used as cross-linking agents, ochratoxin A is used as a template, benzoin dimethyl ether is used as an initiator, N,N-dimethylformamide and PEG 10000 are used as binary pore-foaming agents, and OTA nucleicacid aptamers are used as raw materials, wherein free radical photo-initiation polymerization reaction is carried out to directly generate the aptamer-imprinted monolithic column. The detection recovery rate of the aptamer-imprinted monolithic column for ochratoxin A in a beer matrix reaches 95.5%. The invention provides a new method for specific recognition and separation of ochratoxin A. 本发明公开了特异性识别赭曲霉毒素A的核酸适配体-印迹整体柱及其制