Dynamic cell culture method and device for simulating dynamic environment in vivo

• Main Authors: JIAO YONGJIE, WANG LU, WANG FUJUN, LI CHAOJING, MAO JIFU
• Format: Patent
• Language: Chinese; English
• Published: 17.12.2019
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The invention relates to a dynamic cell culture method and device for simulating the dynamic environment in vivo. The dynamic cell culture method comprises the steps that cells are attached to a flexible substrate in vitro for culture, a cyclic dynamic load with a certain frequency and a certain direction is applied to the flexible substrate in the culture process, in all cyclic periods, the loadis increased gradually along with the time first until reaches a largest value, and then is gradually decreased along with the time, and the deformation rate of the flexible substrate varies with theload synchronously, is increased linearly first, and then is decreased linearly; the culture device comprises a cell culture room, a clamping device for clamping the flexible substrate, a pressing head for applying the load to the flexible substrate, and a center aligning type crank sliding block mechanism for driving the pressing head to make reciprocating motion in the direction perpendicular tothe flexible substrate, an