Umbilical cord Wharton's jelly mesenchymal stem cell osteogenic directional differentiation method

• Main Authors: LIU XIAOCUI, CHU YIFAN, ZHAO LAN, LI JINGJING, JIANG JIAHAO, SUN CANXING, LIANG GUANGRONG
• Format: Patent
• Language: Chinese; English
• Published: 13.12.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides an umbilical cord Wharton's jelly mesenchymal stem cell osteogenic directional differentiation method and relates to the fields of stem cells and regenerative medicines. The method comprises the following steps of tissue digestion, primary culture, subculture, induction culture, etc.; in the tissue digestion step, tissue digestion liquid is used for tissue digestion, whereinthe tissue digestion liquid comprises collagenase I, DNA enzyme and Tryple; and in the induction culture step, an osteoblast differentiation induction culture medium is used for the induction culture, and the osteoblast differentiation induction culture medium comprises an alpha-MEM base bottom solution, a serum substitute, dexamethasone, beta-glycerophosphate, antibiotics, cytokine IL-beta, ascorbic acid and isoflavone. The method can obviously improve induction differentiation efficiency of umbilical cord mesenchymal stem cell osteoblasts. 本发明提供一种脐带华通氏胶间充质干细胞成骨定向分化的方法，涉及干细胞与再生医学领域。该方法，包括组织消化、初代培养、传代培养和诱导培养等步骤；组织消化步